A multipurpose instrument for time-resolved ultra-small-angle and coherent X-ray scattering

High-pressure instrument for small- and wide-angle x-ray scattering. II. Time-resolved experiments

An instrument to facilitate smalland wide-angle x-ray scattering measurements of samples under elevated hydrostatic pressures or exposed to pressure jumps is described. Pressure from atmospheric up to 0.35 GPa is produced by a motor-driven, piston-type generator and transferred through a network containing pressurizing liquid to a sample cell. The cell, with the optical path length of 1.6 mm, h...

Ultra-small-angle X-ray Scattering

Application of small angle X-ray scattering (SAXS) for differentiation between normal and cancerous breast tissues

ABSTRACT Background: Coherent scattering leads to diffraction effects and especially constructive interferences. Theseinterferences carry some information about the molecular structure of the tissue. As breast cancer isthe most widespread cancer in women, this project evaluated the application of small angleX-ray scattering (SAXS) for differentiation between normal and cancerous breast tissues....

Time resolved collapse of a folding protein observed with small angle x-ray scattering.

High-intensity, "pink" beam from an undulator was used in conjunction with microfabricated rapid-fluid mixing devices to monitor the early events in protein folding with time resolved small angle x-ray scattering. This Letter describes recent work on the protein bovine beta-lactoglobulin where collapse from an expanded to a compact set of states was directly observed on the millisecond time sca...

Multistage collapse of a bacterial ribozyme observed by time-resolved small-angle X-ray scattering.

Ribozymes must fold into compact, native structures to function properly in the cell. The first step in forming the RNA tertiary structure is the neutralization of the phosphate charge by cations, followed by collapse of the unfolded molecules into more compact structures. The specificity of the collapse transition determines the structures of the folding intermediates and the folding time to t...